{"id":3926,"date":"2023-11-21T05:43:31","date_gmt":"2023-11-21T10:43:31","guid":{"rendered":"https:\/\/dirtybucketbrewing.com\/?p=3926"},"modified":"2023-11-21T10:42:11","modified_gmt":"2023-11-21T15:42:11","slug":"back-yard-yeast-experiment-building-my-yeast-counts","status":"publish","type":"post","link":"https:\/\/dirtybucketbrewing.com\/?p=3926","title":{"rendered":"Back Yard Yeast Experiment: Building my yeast counts"},"content":{"rendered":"\n

So I harvested some yeast from my back yard, you can read about that here<\/a>. After harvesting I had put together some plates per Bootleg biology<\/a>. The plates were pretty easy to put together and one thing I couldn’t do is sterilizing with a pressure cooker. I’m at a point right now where I’m just playing around and if I ever get really serious into keeping my own yeast strain I’ll definitely look into a pressure cooker for sterilizing. I thing I had learned was keep the extra agar solution and place in a jar. I can scope that out and dilute it later to help grow the colony. Oh well, I tossed it this time.<\/p>\n\n\n\n

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After the plates cooled I had taken a sample of the backyard yeast and streaked the plate. I did this 4 separate times on the same plate. Allowed a few days to pass and poof, we have growth. From this I had pulled another plate and took one sample off the original plate and streaked it again to really insure I have a single strain. I did look under a microscope but it was so packed not sure what I was really looking at. <\/p>\n\n\n\n

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After a few it was finally time to start building up a colony of yeast. I had pulled together about 10mL of 1.020 wort and after sterilizing my loop with the flame I pulled a small yeast sample off the second plate and placed it into the 10mL of wort and set aside, keeping the lid slightly ajar so pressure doesn’t building during fermentation. After about a day I started to see some activity in the 10mL test tube and after 3 days it pretty much stopped. I had given the, now beer a good shake to ensure all the yeast is in solution and created a 100mL of 1.020 wort to start building up the colony more. <\/p>\n\n\n\n

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Activity kicked right up and continued for a few days and finally slowed down. Stepped up the yeast starter with 600mL of 1.040 wort and let that ferment out for a few days. After that now its ready for a small batch. Was going to prop up for a 5 gallon(18L) batch.<\/p>\n\n\n\n

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<\/p>\n","protected":false},"excerpt":{"rendered":"

So I harvested some yeast from my back yard, you can read about that here. After harvesting I had put together some plates per Bootleg biology. The plates were pretty easy to put together and one thing I couldn’t do is sterilizing with a pressure cooker. I’m at a point right now where I’m just […]<\/p>\n","protected":false},"author":1,"featured_media":3936,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[6,341],"tags":[],"class_list":["post-3926","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-misc","category-my-yeast-ex"],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/11\/PXL_20231110_214440105-scaled.jpg?fit=1928%2C2560&ssl=1","jetpack-related-posts":[{"id":4251,"url":"https:\/\/dirtybucketbrewing.com\/?p=4251","url_meta":{"origin":3926,"position":0},"title":"Playing with Yeast brew day 2-25-2024","author":"flintman","date":"April 7, 2024","format":false,"excerpt":"So as I was looking at some ingredients for another brew I decided to take some grain I had from a kit that opened up during shipping and use that to make wort for some yeast starters. I'm going to play around with my frozen yeast and also my backyard\u2026","rel":"","context":"In "My Yeast Experiment"","block_context":{"text":"My Yeast Experiment","link":"https:\/\/dirtybucketbrewing.com\/?cat=341"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2024\/02\/pxl_20240225_1434283692708899505980070602.jpg?fit=904%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2024\/02\/pxl_20240225_1434283692708899505980070602.jpg?fit=904%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2024\/02\/pxl_20240225_1434283692708899505980070602.jpg?fit=904%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2024\/02\/pxl_20240225_1434283692708899505980070602.jpg?fit=904%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]},{"id":3983,"url":"https:\/\/dirtybucketbrewing.com\/?p=3983","url_meta":{"origin":3926,"position":1},"title":"UPDATE: Back Yard Yeast Experiment: Brewing the beer","author":"flintman","date":"December 3, 2023","format":false,"excerpt":"So the beer ended up fermenting rather quickly and actively and down to 1.010SG which give me about a 4.33% beer. Transferred it to a small 1 gallon keg and carbonated up the beer. After carbonation I took a sample after running off a few glasses and it was pretty\u2026","rel":"","context":"In "Brew Session Updates"","block_context":{"text":"Brew Session Updates","link":"https:\/\/dirtybucketbrewing.com\/?cat=337"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/12\/PXL_20231202_180354699-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/12\/PXL_20231202_180354699-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/12\/PXL_20231202_180354699-scaled.jpg?fit=904%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/12\/PXL_20231202_180354699-scaled.jpg?fit=904%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]},{"id":3831,"url":"https:\/\/dirtybucketbrewing.com\/?p=3831","url_meta":{"origin":3926,"position":2},"title":"Back Yard Yeast Experiment: Harvesting my house yeast Take 1….","author":"flintman","date":"October 31, 2023","format":false,"excerpt":"So like the title stated, I'm trying to harvest a yeast strain that ferments wort and also that taste good. I have spent the last year reading about this. I have also been doing some yeast experiments, freezing, reusing, etc to get me ready for this very task. One thing\u2026","rel":"","context":"In "Misc"","block_context":{"text":"Misc","link":"https:\/\/dirtybucketbrewing.com\/?cat=6"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/10\/wp-1697814139095-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/10\/wp-1697814139095-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/10\/wp-1697814139095-scaled.jpg?fit=904%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/10\/wp-1697814139095-scaled.jpg?fit=904%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]},{"id":3956,"url":"https:\/\/dirtybucketbrewing.com\/?p=3956","url_meta":{"origin":3926,"position":3},"title":"Back Yard Yeast Experiment: Brewing the beer","author":"flintman","date":"November 21, 2023","format":false,"excerpt":"Ok, Not that I have collected the yeast and isolated it, check that out here. From that we started with a small yeast starter and built it up for this batch, check that out here. Now its ready to see what this beer can do. I wanted to start off\u2026","rel":"","context":"In "Brewing Sessions"","block_context":{"text":"Brewing Sessions","link":"https:\/\/dirtybucketbrewing.com\/?cat=4"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/11\/PXL_20231121_125853504-1-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/11\/PXL_20231121_125853504-1-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/11\/PXL_20231121_125853504-1-scaled.jpg?fit=904%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2023\/11\/PXL_20231121_125853504-1-scaled.jpg?fit=904%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]},{"id":881,"url":"https:\/\/dirtybucketbrewing.com\/?p=881","url_meta":{"origin":3926,"position":4},"title":"MISC – Freezing Yeast","author":"flintman","date":"January 20, 2021","format":false,"excerpt":"So as most people know I'm a frugal person. With that said when I brew a batch of beer it can cost be from $25-$50+ per 5 gallon batch. Looking at the ingredients that go into making beer and keeping my cost down, I looked at yeast being the best\u2026","rel":"","context":"In "My Yeast Experiment"","block_context":{"text":"My Yeast Experiment","link":"https:\/\/dirtybucketbrewing.com\/?cat=341"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/01\/IMG_20201212_150424.jpg?fit=900%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/01\/IMG_20201212_150424.jpg?fit=900%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/01\/IMG_20201212_150424.jpg?fit=900%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/01\/IMG_20201212_150424.jpg?fit=900%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]},{"id":1585,"url":"https:\/\/dirtybucketbrewing.com\/?p=1585","url_meta":{"origin":3926,"position":5},"title":"Freeze your Yeast update","author":"flintman","date":"February 14, 2022","format":false,"excerpt":"Back about a year now, a few days over, I had posted about Freezing Yeast , now since I had done this I haven't used that yeast to much as I was brewing many different styles. With that said today I was thinking, Hmm I should see if that frozen\u2026","rel":"","context":"In "My Yeast Experiment"","block_context":{"text":"My Yeast Experiment","link":"https:\/\/dirtybucketbrewing.com\/?cat=341"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/02\/PXL_20220212_004823567-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/02\/PXL_20220212_004823567-scaled.jpg?fit=904%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/02\/PXL_20220212_004823567-scaled.jpg?fit=904%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/dirtybucketbrewing.com\/wp-content\/uploads\/2022\/02\/PXL_20220212_004823567-scaled.jpg?fit=904%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]}],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/posts\/3926","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3926"}],"version-history":[{"count":7,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/posts\/3926\/revisions"}],"predecessor-version":[{"id":3954,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/posts\/3926\/revisions\/3954"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=\/wp\/v2\/media\/3936"}],"wp:attachment":[{"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3926"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3926"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dirtybucketbrewing.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3926"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}